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Re: Problem of software compensation with Flowjo 6.0

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From: Adam Treister <mailto:adam@treestar.com?subject=Re:%20Problem%20of%20software%20compensation%20with%20Flowjo%206.0> Date: Sat Feb 19 2005 - 00:49:34 EST
Alex,

The feature changed slightly in the 6.0 release. Now, the compensation
matrix is by default saved within the workspace, instead of in a
separate file. If you need to export the file (as is the only option
in the older versions), there is an additional menu item for Edit /
Save Matrix, under Platform >> Compensate Sample.

Adam


On Feb 18, 2005, at 6:08 AM, Li Chen wrote:

> Hi Flowers,
>
> Sometimes it is not easy to do the hardware compensation when
> dealing with very small number of cells. I collect my FACS data with
> BDCalibur and try to do software compensation using FlowJo 6.0 on Mac
> (OS 9.0). I followed the manual step by step: define the matrix using
> single color sample--> save the matrix --> load the matrix--> apply it
> to my samples of interest. What troubles me is that after I saved the
> matrix I couldn't load it. I couldn't find its location using the
> search function in the computer although it appeared on the manual
> under
> COMPENSATION BAR. I wonder if it is a bug for this software (I have no
> problem with Window system using the demo data). Does anyone out there
> have some ideas?
>
> Alex

---------------------------------------------
Adam Treister
Tree Star, Inc.

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Received on Mon Feb 21 14:18:00 2005
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